Do government regulations "kill jobs"?

Yes, but they also create jobs:

"If you’re a coal miner in West Virginia, it’s not a great comfort that a bunch of guys in Texas are employed doing natural gas," said Roger Noll, an economics professor at Stanford and co-director of the university’s program on regulatory policy. "Some people identify with the beneficiaries, others identify with those who bear the cost, and no amount of argument is ever going to change their minds."

And to the extent we can trust self-reporting by employers, it seems that not many jobs are killed by regulations:

Data from the Bureau of Labor Statistics show that very few layoffs are caused principally by tougher rules.

Whenever a firm lays off workers, the bureau asks executives the biggest reason for the job cuts.

In 2010, 0.3 percent of the people who lost their jobs in layoffs were let go because of “government regulations/intervention.” By comparison, 25 percent were laid off because of a drop in business demand.